Gamma-Radiation Induced Endoreplication in Exposed CHO Cell Line
نویسنده
چکیده
s: Several studies have highlighted the possibility of radiation induced chromosomal aberrations. The present study explains the susceptibility of numerical and structural chromosomal alterations, specifically endoreplication in irradiated Chinese Hamster Ovary (CHO) cell line. A total of 426 metaphase spreads prepared from CHO cells, exposed to 2 or 6 Gy of Co -rays were analyzed. The frequency of dicentric chromosomes 60 was 48 %, endoreplications 12.6 % and acenteric fragments 12.7 %. The manifestation of endoreplication after exposure to ionizing radiation in comparison with control cells was significant. Also, dicentric chromosomes were appeared to be created in a dose-dependent manner and observed more frequently than acentric fragments. However, the dose-dependent effect of irradiation was not seen in the case of endoreplication. This study suggests the possible hazardous effect of radiation on spindle function, microtubule motor or structure, resulting in endoreplication.
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